| Description | Human DCC knockout HCT 15 cell line is HCT 15 cell line and it is edited by our special CRISPR/Cas9 technology. HCT 15 is a human colon cancer cell line used for therapeutic research and drug screening. HCT116 cells are also used in various biomedical studies involving colon cancer proliferation and corresponding inhibitors |
| Size | 1 vial (2*106 cells/vial) |
| Parental Cell Line | HCT 15 |
| Biosafety Level | 1 |
| Format | Frozen |
| Culture Conditions | Culture at 5% CO2, 37℃ |
| Culture Medium | RPMI 1640 + 10% FBS + 2mM L-Glutamine |
| Subculture Conditions | Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4*104 cells/cm2. |
| Freezing Medium | Culture medium + 50% FBS + 10% DMSO |
| Knockout Validation | Sanger Sequencing, Western Blot (WB) |
| Viability | >95% viability before freezing. |
| Adherent /Suspension | Adherent |
| Disease | Adenocarcinoma; Colorectal; Dukes' type C |
| Purity | Immunogen affinity purified |
| Storage | Vapor phase of liquid nitrogen |
| Sterility | Bacteria: Negative Yeast: Negative Mycoplasma: Negative |
| Shipping Info | Dry Ice |
| Species | Human |
| Tested Applications | Suitable for: ICC, WB |
| Tissue | Colon |
| Recommended Control | Human wild-type HCT 15 cell line |
| Morphology | Epithelial |
| Pathogens | HIV: Negative Hepatitis B: Negative Hepatitis C: Negative |
| Cytogenic Data | A quasidiploid human cell line |
| Tumorigenicity | Yes, in nude mice. |
| Applications | 3D cell culture High-throughput screening Toxicology |
| Product Type | Knockout Stable Cell Line |
| Price | Inquiry |
