| Description | H295RA is an ACTH-responsive human adrenocortical cell line, produced from H295R cells that have been transduced to stable express increased human MRAP (MC2R accessory protein) gene. H295R adrenocortical cell line displays relatively low ACTH responsiveness due to low melanocortin 2 receptor (MC2R) expression. Successful induction of MRAP and increased expression of MC2R in H295RA cells was confirmed by quantitative real-time RT-PCR and protein analysis. This cell line is well-suited for research regarding molecular analysis of physiologic and pathologic conditions involving the hypothalamic–pituitary–adrenal axis. |
| Species | Human |
| Donor Disease | Adrenocortical Carcinoma |
| Disease Subtype | Adrenocortical Carcinoma |
| Donor Gender | Female |
| Donor Age | 48 years old |
| Donor Ethnicity | African American |
| Tissue/Organ/Organ System | Adrenal Gland |
| Immortalization Method | Spontaneous |
| Cell Morphology | Polygonal |
| Population Doubling Time | 36 - 48 hours |
| Expression Profile | Transgenic expression of human MRAP; ACTH responsive steroid production and enzyme induction; angiotensin II responsive aldosterone production and aldosterone synthase expression. Selection marker: blasticidin 1ug/mL. Vector: pLX 304-Blast-V5 (Accession #BC062721) |
| Quality Control | 1) Monoclonal selection using cloning cylinders; 2) quantitative real-time PCR; 3) Protein analysis |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Application | For Research Use Only |
| Price | Inquiry |
- HOME
- About Us
- Cell Lines
- Custom Knockout Cell Lines
- Drug Discovery Cell Lines
- Immortalized Cells
- Adipose
- Airway/Lung
- Auditory
- Bladder
- Blood
- Blood Vessel
- Bone
- Bone Marrow
- Brain
- Breast
- Colon
- Digestive
- Embryo
- Eye
- Hair
- Hair Follicle
- Heart
- Intestinal
- Kidney
- Liver
- Lung
- Mammary
- Mesentery
- Oral
- Ovary
- Pancreas
- Pituitary Gland
- Prostate
- Renal
- Reproductive
- Skeletal
- Skeletal Muscle
- Skin
- Spleen
- Stomach
- Straitum
- Testis
- Thyroid
- Primary Cells
- By Cell Type
- Adipocytes
- Astrocyte
- Cardiomyocyte
- Chondrocyte
- Dendritic
- Dermal Papilla Cells
- Endothelial
- Epithelial
- Fibroblast
- Interstitial
- Keratinocytes
- Keratocyte
- Kupffer
- Lymphocyte
- Macrophage
- Melanocyte
- Microglia
- Monocyte
- Mononuclear
- Neuron
- Osteoblast
- Preadipocytes
- Schwann Cells
- Skeletal Muscle
- Stellate
- Synoviocyte
- Trabecular Meshwork Cells
- Vascular Smooth Muscle Cells
- By Species Type
- By Tissue Type
- Adipose
- Airway
- Airway/Lung
- Auditory
- Blood
- Blood Vessel
- Bone
- Bone Marrow
- Brain
- Breast
- Cardiac
- Colon
- Digestive
- Eye
- Fat
- Gastrointestinal
- Hair
- Hair Follicle
- Heart
- Joint
- Kidney
- Liver
- Mammary
- Microvascular
- Muscle
- Nervous
- Oral
- Peritoneum
- Placenta
- Renal
- Reproductive
- Skeletal Muscal
- Skin
- Spleen
- Thyroid
- Umbilical
- Urogenital
- By Cell Type
- Stable Cell Lines
- Apoptosis Assay Cell Lines
- CRE Expression Cell Lines
- CRE Reporter Cell Lines
- CRISPR Ready Cell Lines
- Fluorescent Cell Lines
- GPCRs Expressing Cell Lines
- GPCRs Internalization Assay Cell Lines
- Immortalized Primary Cells
- Inducible Expression Cell Lines
- Ion Channel Cell Lines
- LacZ Cell Lines
- Luciferase Cell Lines
- Multiplexing GPCR Screening Cell Lines - NOMAD
- Neurodegenerative Disease Model Cell Line for Screening
- Other Stable Cell Lines
- Pathway Cell Lines
- Premade Labeled Cancer Cell Lines
- rtTA Cell Lines
- Stable Cell Lines for CNS Research
- Stable Cell Lines for Drug Screening in CNS
- TetR Cell Lines
- Transfected Stable Cell Lines
- Stem Cells
- Tumor Cells
- By Tissue Type
- Adrenal Gland
- Airway/Lung
- Ampulla of Vater
- Bile Duct
- Biliary Tract
- Bladder/Urinary Tract
- Blood
- Bone
- Bone Marrow
- Bowel
- Brain
- Breast
- Cervix
- CNS/Brain
- Colon
- Digestive
- Esophagus/Stomach
- Eye
- Head and Neck
- Kidney
- Larynx
- Liver
- Lung
- Lymphoid
- Mammary
- Myeloid
- Oral
- Other
- Ovary/Fallopian Tube
- Pancreas
- Peripheral Nervous System
- Pleura
- Prostate
- Renal
- Reproductive
- Skeletal
- Skin
- Soft Tissue
- Spleen
- Stomach
- Testis
- Thymic
- Thymus
- Thyroid
- Uterus
- Vulva/Vagina
- By Tissue Type
- Services
- Company
