| Description | Human AC005594.3 knockout KBM-7 cell line was drived from KBM-7. KBM-7 is a near-haploid human chronic granulocytic leukemia (CGL) cell line commonly used in biomedical research. KBM-7 cells, like all immortal cancer cell lines, divide indefinitely, however, this cell line is unique in that it contains only one copy. |
| Size | 1 vial (2*106 cells/vial) |
| Parental Cell Line | KBM-7 |
| Biosafety Level | 1 |
| Format | Frozen |
| Culture Conditions | Cultured at 37°C with 5% CO2. |
| Culture Medium | Iscove's Modified Dulbecco's Medium (IMDM) supplemented with 10% fetal bovine serum. |
| Subculture Conditions | All seeding densities should be based on cell counts obtained by established methods. The recommended seeding density is 2*106 cells/cm2. |
| Derived From | Bone marrow |
| Freezing Medium | IMDM, 20% FBS, 10% DMSO |
| Subculture Guidelines | All seeding densities should be based on cell counts obtained by established methods. The recommended seeding density is 2*106 cells/cm2. |
| Knockout Validation | Sanger Sequencing, Western Blot (WB) |
| Viability | >95% viability before freezing. |
| Adherent /Suspension | Adherent |
| Disease | Chronic myeloid leukemia |
| Purity | Immunogen affinity purified |
| Storage | Liquid nitrogen |
| Sterility | Bacteria: Negative Yeast: Negative Mycoplasma: Negative |
| Shipping Info | Dry Ice |
| Species | Human |
| Tested Applications | Suitable for: WB, Sanger Sequencing |
| Recommended Control | Human wild-type KBM-7 cell line |
| Doubling Time | 22 hours |
| Pathogens | HIV: Negative Hepatitis B: Negative Hepatitis C: Negative |
| Karyotypic Information | Haploid except for a disomy of chromosome 8. |
| Applications | A variety of genomic research studies |
| Product Type | Knockout Stable Cell Line |
| Price | Inquiry |
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