| Description | Human AKT1 knockout ZR-75-1 cell line is edited by our special CRISPR/Cas9 technology. ZR-75-1 is a human breast cancer cell line |
| Size | 1 vial (2*106 cells/vial) |
| Parental Cell Line | ZR-75-1 |
| Biosafety Level | 1 |
| Fromat | Frozen |
| Culture Conditions | Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. |
| Subculture Conditions | 1:6 subculture; feed cells 2 to 3 times per week. 5% CO2 culture; 37℃ |
| Freezing Medium | Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. |
| Subculture Guidelines | All seeding densities should be based on cell counts obtained by established methods. The recommended seeding density is 2*104 cells/cm2. If necessary, a partial replacement of the medium 24 hours before subculture may help promote growth. When the cells achieve 80-90% confluence, they should be passaged. |
| Knockout Validation | Sanger Sequencing |
| Viability | >95% viability before freezing. |
| Adherent /Suspension | Adherent |
| Disease | Ductal carcinoma |
| Purity | Immunogen affinity purified |
| Storage | Vapor phase of liquid nitrogen |
| Sterility | Bacteria: Negative Yeast: Negative Mycoplasma: Negative |
| Applications | For research use only |
| Shipping Info | Dry Ice |
| Species | Human |
| Tissue | Breast; Duct; Mammary gland |
| Recommended Control | Human wild-type ZR-75-1 cell line |
| Morphology | Epithelial cell |
| Karyotype | The cell line has a hypertriploid chromosome number. |
| Pathogens | HIV: Negative Hepatitis B: Negative Hepatitis C: Negative |
| Product Type | Knockout Stable Cell Line |
| Price | Inquiry |
