| Description | Human B2M knockout MDA-MB-157 cell line is edited by our special CRISPR/Cas9 technology. MDA-MB-157 was established from the pleural effusion of a 44-year-old woman with metastatic breast cancer. Desmosomes, microvilli and tension filaments are observed at the border between cells |
| Size | 1 vial (2*106 cells/vial) |
| Parental Cell Line | MDA-MB-157 |
| Biosafety Level | 1 |
| Format | Frozen |
| Culture Conditions | Culture at 100% Air, 37℃ |
| Culture Medium | L15 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS). |
| Subculture Conditions | A subcultivation ratio of 1:3 to 1: 6 is recommended using 0.25% trypsin or trypsin/EDTA. |
| Derived From | Pleural effusion |
| Freezing Medium | Complete culture medium supplemented with 5% (v/v) DMSO |
| Subculture Guidelines | This cell line is relatively slow growing and should be left 5-7 days between splits and the culture media should be changed every 2-3 days. When resuscitating from frozen seed into non-vented flasks at 4-5*10,000 cells/cm2. Split sub-confluent cultures (80-90 %) 1:3 to 1:6, i.e. seeding at 1-3 x10,000 cells/cm²; 37℃, Air. |
| Knockout Validation | Sanger Sequencing |
| Viability | >95% viability before freezing. |
| Adherent /Suspension | Adherent |
| Disease | Medulallary carcinoma |
| Purity | Immunogen affinity purified |
| Storage | Vapor phase of liquid nitrogen |
| Sterility | Mycoplasma negative, HOECHST and PCR |
| Shipping Info | Dry Ice |
| Species | Human |
| Tested Applications | Suitable for: ICC, WB, Sanger Sequencing |
| Tissue | Mammary gland; breast/medulla |
| Recommended Control | Human wild-type MDA-MB-157 cell line |
| Morphology | Epithelial |
| Pathogens | HIV: Negative Hepatitis B: Negative |
| Cytogenic Data | Knockout Stable Cell Line |
| Tumorigenicity | In nude mice and in immunosuppressed BALB/c mice. |
| Applications | For research use only |
| Product Type | Knockout Stable Cell Line |
| Price | Inquiry |
