| Description | Human B2M knockout MDA-MB-468 cell line is edited by our special CRISPR/Cas9 technology. MDA-MB-468 is a cell line isolated in 1977 from a 51-year-old female human and is commonly used in the studies of metastasis, migration, and breast cancer proliferation. |
| Size | 1 vial (2*106 cells/vial) |
| Parental Cell Line | MDA-MB-468 |
| Biosafety Level | 1 |
| Format | Frozen |
| Culture Conditions | Cells are adherent cells that are cultured at 37℃ in a humidified atmosphere with 5% CO2. |
| Culture Medium | DMEM + 10% FBS + 2mM L-Glutamine; split cultures 1:2-1:4 using trypsin/EDTA |
| Properties | Estrogen receptor alpha negative. HLA: Aw23, Aw30; B27, Bw35; Cw2, Cw4 |
| Subculture Conditions | Split cultures 1:2-1:4 using trypsin/EDTA |
| Derived From | Pleural effusion |
| Freezing Medium | Culture medium + 50% FBS + 10% DMSO |
| Subculture Guidelines | All seeding densities should be based on cell counts obtained by established methods. Seed at 2-4*104cells/cm2; 37C, 5% CO2; do not allow to reach confluence |
| Knockout Validation | Sanger Sequencing |
| Viability | >95% viability before freezing. |
| Adherent /Suspension | Adherent |
| Disease | Adenocarcinoma |
| Purity | Immunogen affinity purified |
| Storage | Vapor phase of liquid nitrogen |
| Sterility | Mycoplasma negative, HOECHST and PCR |
| Shipping Info | Dry Ice |
| Species | Human |
| Tested Applications | Suitable for: ICC, WB, Sanger Sequencing |
| Tissue | Breast |
| Recommended Control | Human wild-type MDA-MB-468 cell line |
| Doubling Time | ~36-62 h |
| Morphology | Epithelial-like, grown as monolayer |
| Cytogenic Data | Modal number = 64; range = 60 to 67. |
| Tumorigenicity | Tumorigenic in nude mice |
| Applications | For research use only |
| Product Type | Knockout Stable Cell Line |
| Price | Inquiry |
