Name: Human BAX knockout MCF7 cell line
SKU: SCELL-37687

Human BAX knockout MCF7 cell line

Description	Human BAX knockout MCF7 cell line is edited by our special CRISPR/Cas9 technology.
Size	1 vial (2*10⁶ cells/vial)
Parental Cell Line	MCF7
Biosafety Level	1
Fromat	Frozen
Culture Conditions	Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2.
Subculture Conditions	1:2 to 1:6 (80-90%) using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37℃
Derived From	Pleural effusion
Freezing Medium	Continuous culture; DMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:2-1:6 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2. 37℃, 5% CO2.
Subculture Guidelines	All seeding densities should be based on cell counts obtained by established methods. The recommended seeding density is 2*10⁴ cells/cm². If necessary, a partial replacement of the medium 24 hours before subculture may help promote growth. When the cells achieve 80-90% confluence, they should be passaged.
Knockout Validation	Sanger Sequencing
Viability	>95% viability before freezing.
Adherent /Suspension	Adherent
Disease	Adenocarcinoma
Purity	Immunogen affinity purified
Storage	Vapor phase of liquid nitrogen
Sterility	Mycoplasma negative，PCR
Applications	For research use only
Shipping Info	Dry Ice
Species	Human
Tissue	Breast
Recommended Control	Human wild-type MCF7 cell line
Doubling Time	Ca. 50 hours (range 30-72 hours)
Morphology	Epithelial-like, grown as monolayer
Original Line	MCF7
Pathogens	HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Product Type	Knockout Stable Cell Line
Price	Inquiry