| Description | Human BCL11B knockout HCT 116 cell line is edited by our special CRISPR/Cas9 technology. HCT 116 is a human colon cancer cell line used for therapeutic research and drug screening. HCT116 cells are also used in various biomedical studies involving colon cancer proliferation and corresponding inhibitors |
| Size | 1 vial (2*106 cells/vial) |
| Parental Cell Line | HCT 116 |
| Biosafety Level | 1 |
| Format | Frozen |
| Culture Conditions | Culture at 5% CO2, 37℃ |
| Culture Medium | McCoy's 5a + 2mM Glutamine + 10% FBS |
| Subculture Conditions | Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA |
| Derived From | Established from the primary colon carcinoma |
| Freezing Medium | Complete culture medium supplemented with 5% (v/v) DMSO |
| Knockout Validation | Immunocytochemistry (ICC), Sanger Sequencing, Western Blot (WB) |
| Viability | >95% viability before freezing. |
| Adherent /Suspension | Adherent |
| Disease | Colorectal carcinoma |
| Purity | Immunogen affinity purified |
| Storage | Vapor phase of liquid nitrogen |
| Sterility | Bacteria: Negative Yeast: Negative Mycoplasma: Negative |
| Shipping Info | Dry Ice |
| Species | Human |
| Tested Applications | Suitable for: ICC, WB |
| Tissue | Colon |
| Recommended Control | Human wild-type HCT 116 cell line |
| Doubling Time | ~25-48 h |
| Morphology | Epithelial-like morphology |
| Pathogens | HIV: Negative Hepatitis B: Negative Hepatitis C: Negative |
| Cytogenic Data | Tumorigenic; the stemline chromosome number is near diploid |
| Tumorigenicity | Yes, in nude mice. |
| Applications | For research use only |
| Product Type | Knockout Stable Cell Line |
| Price | Inquiry |
