| Description | Human BMPR1A knockout DLD-1 cell line is DLD-1 cell line and it is edited by our special CRISPR/Cas9 technology. DLD-1 is human Dukes's type C colorectal adenocarcinoma cell line, which was originally one of two colorectal adenocarcinoma cell lines isolated between 1977 and 1979. |
| Size | 1 vial (2*106 cells/vial) |
| Parental Cell Line | DLD-1 |
| Biosafety Level | 1 |
| Format | Frozen |
| Culture Conditions | Culture at 5% CO2, 37℃ |
| Culture Medium | RPMI 1640 + 2mM Glutamine + 10% FBS |
| Subculture Conditions | 0.25% trypsin, 0.02% EDTA |
| Freezing Medium | Culture medium + 50% FBS + 10% DMSO |
| Subculture Guidelines | All seeding densities should be based on cell counts obtained by established methods. The recommended seeding density is at seeding at 1-3*10,000 cells/cm2 |
| Knockout Validation | Immunocytochemistry (ICC), Sanger Sequencing, Western Blot (WB) |
| Viability | >95% viability before freezing. |
| Adherent /Suspension | Adherent |
| Disease | Adenocarcinoma; Colorectal; Dukes' type C |
| Purity | Immunogen affinity purified |
| Storage | Vapor phase of liquid nitrogen |
| Sterility | Bacteria: Negative Yeast: Negative Mycoplasma: Negative |
| Shipping Info | Dry Ice |
| Species | Human |
| Tested Applications | Suitable for: ICC, WB |
| Tissue | Large intestine; Colon |
| Recommended Control | Human wild-type DLD-1 cell line |
| Morphology | Epithelial-like, grown as monolayer |
| Pathogens | HIV: Negative Hepatitis B: Negative Hepatitis C: Negative |
| Cytogenic Data | This is a pseudodiploid human cell line |
| Tumorigenicity | Tumorigenic in nude mice |
| Applications | 3D cell culture Cancer research |
| Product Type | Knockout Stable Cell Line |
| Price | Inquiry |
