| Description | Human BRCA1 knockout A549 cell line is edited by our special CRISPR/Cas9 technology. A549 cells are squamous in nature and are responsible for the diffusion of water and electrolytes in the alveoli. When grown in vitro, these cells grow as a monolayer, adhering to the culture flask |
| Size | 1 vial (2*106 cells/vial) |
| Parental Cell Line | A549 |
| Biosafety Level | 1 |
| Fromat | Frozen |
| Culture Conditions | Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. |
| Freezing Medium | Continuous culture; DMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:4 using trypsin/EDTA; |
| Subculture Guidelines | All seeding densities should be based on cell counts obtained by established methods. The recommended seeding density is 2*104 cells/cm2. If necessary, a partial replacement of the medium 24 hours before subculture may help promote growth. When the cells achieve 80-90% confluence, they should be passaged. |
| Knockout Validation | Sanger Sequencing |
| Viability | >95% viability before freezing. |
| Adherent /Suspension | Adherent |
| Disease | Adenocarcinoma |
| Purity | Immunogen affinity purified |
| Storage | Vapor phase of liquid nitrogen |
| Sterility | Mycoplasma negative, HOECHST and PCR |
| Applications | For research use only |
| Shipping Info | Dry Ice |
| Species | Human |
| Tissue | Lung |
| Recommended Control | Human wild-type A549 cell line |
| Doubling Time | A549 cell doubling time is typically 22 hours, but can sometimes take up to 40 hours |
| Morphology | Epithelial, grown as monolayer |
| Karyotype | Hypotriploid |
| Pathogens | HIV: Negative Hepatitis B: Negative Hepatitis C: Negative |
| Cell Size | In the morphological analysis of A549 cells, the mean cell diameters from an inverted microscope and TEM images were estimated to be 14.93 µm and 10.59 µm, respectively. |
| Product Type | Knockout Stable Cell Line |
| Price | Inquiry |
