| Description | Human BRD4 knockout HCT 116 cell line is edited by our special CRISPR/Cas9 technology. HCT 116 is a human colon cancer cell line used for therapeutic research and drug screening. HCT116 cells are also used in various biomedical studies involving colon cancer proliferation and corresponding inhibitors |
| Size | 1 vial (2*106 cells/vial) |
| Parental Cell Line | HCT 116 |
| Biosafety Level | 1 |
| Format | Frozen |
| Culture Conditions | Culture at 5% CO2, 37℃ |
| Culture Medium | McCoy's 5a + 2mM Glutamine + 10% FBS |
| Subculture Conditions | Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA |
| Derived From | Established from the primary colon carcinoma |
| Freezing Medium | Complete culture medium supplemented with 5% (v/v) DMSO |
| Knockout Validation | Sanger Sequencing, Western Blot (WB) |
| Viability | >95% viability before freezing. |
| Adherent /Suspension | Adherent |
| Disease | Colorectal carcinoma |
| Purity | Immunogen affinity purified |
| Storage | Vapor phase of liquid nitrogen |
| Sterility | Bacteria: Negative Yeast: Negative Mycoplasma: Negative |
| Shipping Info | Dry Ice |
| Species | Human |
| Tested Applications | Suitable for: ICC, WB |
| Tissue | Colon |
| Recommended Control | Human wild-type HCT 116 cell line |
| Doubling Time | ~25-48 h |
| Morphology | Epithelial-like morphology |
| Pathogens | HIV: Negative Hepatitis B: Negative Hepatitis C: Negative |
| Cytogenic Data | Tumorigenic; the stemline chromosome number is near diploid |
| Tumorigenicity | Yes, in nude mice. |
| Applications | For research use only |
| Product Type | Knockout Stable Cell Line |
| Price | Inquiry |
- HOME
- About Us
- Cell Lines
- Custom Knockout Cell Lines
- Drug Discovery Cell Lines
- Immortalized Cells
- Adipose
- Airway/Lung
- Auditory
- Bladder
- Blood
- Blood Vessel
- Bone
- Bone Marrow
- Brain
- Breast
- Colon
- Digestive
- Embryo
- Eye
- Hair
- Hair Follicle
- Heart
- Intestinal
- Kidney
- Liver
- Lung
- Mammary
- Mesentery
- Oral
- Ovary
- Pancreas
- Pituitary Gland
- Prostate
- Renal
- Reproductive
- Skeletal
- Skeletal Muscle
- Skin
- Spleen
- Stomach
- Straitum
- Testis
- Thyroid
- Primary Cells
- By Cell Type
- Adipocytes
- Astrocyte
- Cardiomyocyte
- Chondrocyte
- Dendritic
- Dermal Papilla Cells
- Endothelial
- Epithelial
- Fibroblast
- Interstitial
- Keratinocytes
- Keratocyte
- Kupffer
- Lymphocyte
- Macrophage
- Melanocyte
- Microglia
- Monocyte
- Mononuclear
- Neuron
- Osteoblast
- Preadipocytes
- Schwann Cells
- Skeletal Muscle
- Stellate
- Synoviocyte
- Trabecular Meshwork Cells
- Vascular Smooth Muscle Cells
- By Species Type
- By Tissue Type
- Adipose
- Airway
- Airway/Lung
- Auditory
- Blood
- Blood Vessel
- Bone
- Bone Marrow
- Brain
- Breast
- Cardiac
- Colon
- Digestive
- Eye
- Fat
- Gastrointestinal
- Hair
- Hair Follicle
- Heart
- Joint
- Kidney
- Liver
- Mammary
- Microvascular
- Muscle
- Nervous
- Oral
- Peritoneum
- Placenta
- Renal
- Reproductive
- Skeletal Muscal
- Skin
- Spleen
- Thyroid
- Umbilical
- Urogenital
- By Cell Type
- Stable Cell Lines
- Apoptosis Assay Cell Lines
- CRE Expression Cell Lines
- CRE Reporter Cell Lines
- CRISPR Ready Cell Lines
- Fluorescent Cell Lines
- GPCRs Expressing Cell Lines
- GPCRs Internalization Assay Cell Lines
- Immortalized Primary Cells
- Inducible Expression Cell Lines
- Ion Channel Cell Lines
- LacZ Cell Lines
- Luciferase Cell Lines
- Multiplexing GPCR Screening Cell Lines - NOMAD
- Neurodegenerative Disease Model Cell Line for Screening
- Other Stable Cell Lines
- Pathway Cell Lines
- Premade Labeled Cancer Cell Lines
- rtTA Cell Lines
- Stable Cell Lines for CNS Research
- Stable Cell Lines for Drug Screening in CNS
- TetR Cell Lines
- Transfected Stable Cell Lines
- Stem Cells
- Tumor Cells
- By Tissue Type
- Adrenal Gland
- Airway/Lung
- Ampulla of Vater
- Bile Duct
- Biliary Tract
- Bladder/Urinary Tract
- Blood
- Bone
- Bone Marrow
- Bowel
- Brain
- Breast
- Cervix
- CNS/Brain
- Colon
- Digestive
- Esophagus/Stomach
- Eye
- Head and Neck
- Kidney
- Larynx
- Liver
- Lung
- Lymphoid
- Mammary
- Myeloid
- Oral
- Other
- Ovary/Fallopian Tube
- Pancreas
- Peripheral Nervous System
- Pleura
- Prostate
- Renal
- Reproductive
- Skeletal
- Skin
- Soft Tissue
- Spleen
- Stomach
- Testis
- Thymic
- Thymus
- Thyroid
- Uterus
- Vulva/Vagina
- By Tissue Type
- Services
- Company
