| Description | Human CALR knockout BT-549 cell line is edited by our special CRISPR/Cas9 technology. BT-549 is an immortalized human triple-negative breast ductal carcinoma cell line. BT-549 cells are polymorphic, possess epithelial-like components and multinucleated giant cells, and can secrete mucin-like substances into the culture medium, and the cells can be transfected into host cells |
| Size | 1 vial (2*106 cells/vial) |
| Parental Cell Line | BT-549 |
| Biosafety Level | 1 |
| Fromat | Frozen |
| Culture Conditions | Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. |
| Subculture Conditions | Isolate subconfluent cultures (70-80%) at a seed ratio of 1:2 to 1:6 using 0.25% trypsin; 0.05% EDTA. Incubate at 37°C in 5% CO2. Renew the medium every 2 to 3 days. |
| Freezing Medium | Complete culture medium supplemented with 5% (v/v) DMSO |
| Subculture Guidelines | All seeding densities should be based on cell counts obtained by established methods. The recommended seeding density is 2*104 cells/cm2. If necessary, a partial replacement of the medium 24 hours before subculture may help promote growth. When the cells achieve 80-90% confluence, they should be passaged. |
| Knockout Validation | Sanger Sequencing, Western Blot (WB) |
| Viability | >95% viability before freezing. |
| Adherent /Suspension | Adherent |
| Disease | Ductal carcinoma |
| Purity | Immunogen affinity purified |
| Storage | Vapor phase of liquid nitrogen |
| Sterility | Bacteria: Negative Yeast: Negative Mycoplasma: Negative |
| Applications | For research use only |
| Shipping Info | Dry Ice |
| Species | Human |
| Tested Applications | Suitable for: WB |
| Tissue | Breast; Mammary gland |
| Recommended Control | Human wild-type BT-549 cell line |
| Morphology | Epithelial cell |
| Karyotype | This cell line is an aneuploid human female with chromosome counts in the hypertriploid range. |
| Pathogens | HIV: Negative Hepatitis B: Negative Hepatitis C: Negative |
| Product Type | Knockout Stable Cell Line |
| Price | Inquiry |
