| Description | Human CD3E knockout T-47D cell line is edited by our special CRISPR/Cas9 technology. T-47D is a human breast cancer cell line commonly used in biomedical research involving hormone expression. The cells were originally isolated from the pleural effusion of a 54-year-old woman with invasive ductal carcinoma of the breast |
| Size | 1 vial (2*106 cells/vial) |
| Parental Cell Line | T-47D |
| Biosafety Level | 1 |
| Fromat | Frozen |
| Culture Conditions | Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. |
| Subculture Conditions | 1:3 to 1:5 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37℃ |
| Freezing Medium | Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. |
| Subculture Guidelines | All seeding densities should be based on cell counts obtained by established methods. The recommended seeding density is 2*104 cells/cm2. If necessary, a partial replacement of the medium 24 hours before subculture may help promote growth. When the cells achieve 80-90% confluence, they should be passaged. |
| Knockout Validation | Sanger Sequencing |
| Viability | >95% viability before freezing. |
| Adherent /Suspension | Adherent |
| Disease | Breast ductal carcinoma |
| Purity | Immunogen affinity purified |
| Storage | Vapor phase of liquid nitrogen |
| Sterility | Bacteria: Negative Yeast: Negative Mycoplasma: Negative |
| Applications | For research use only |
| Shipping Info | Dry Ice |
| Species | Human |
| Tissue | Breast |
| Recommended Control | Human wild-type T-47D cell line |
| Doubling Time | Ca. 30-40 hours |
| Morphology | Epithelial cell |
| Karyotype | This is a hypotriploid human cell line |
| Estrogen Receptors | Yes |
| Pathogens | HIV: Negative Hepatitis B: Negative Hepatitis C: Negative |
| Product Type | Knockout Stable Cell Line |
| Price | Inquiry |
