| Description | Human CD86 knockout MCF7 490X1 cell line is edited by our special CRISPR/Cas9 technology. |
| Size | 1 vial (2*106 cells/vial) |
| Parental Cell Line | MCF7 490X1 |
| Biosafety Level | 1 |
| Fromat | Frozen |
| Culture Conditions | Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. |
| Subculture Conditions | 1:2 to 1:6 (80-90%) using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37℃ |
| Derived From | Pleural effusion |
| Freezing Medium | Continuous culture; DMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:2-1:6 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2. 37℃, 5% CO2. |
| Subculture Guidelines | All seeding densities should be based on cell counts obtained by established methods. The recommended seeding density is 2*104 cells/cm2. If necessary, a partial replacement of the medium 24 hours before subculture may help promote growth. When the cells achieve 80-90% confluence, they should be passaged. |
| Knockout Validation | Sanger Sequencing, Western Blot (WB) |
| Viability | >95% viability before freezing. |
| Adherent /Suspension | Adherent |
| Disease | Adenocarcinoma |
| Purity | Immunogen affinity purified |
| Storage | Vapor phase of liquid nitrogen |
| Sterility | Mycoplasma negative，PCR |
| Applications | For research use only |
| Shipping Info | Dry Ice |
| Species | Human |
| Tissue | Breast |
| Recommended Control | Human wild-type MCF7 490X1 cell line |
| Doubling Time | Ca. 50 hours (range 30-72 hours) |
| Morphology | Epithelial-like, grown as monolayer |
| Original Line | MCF7 |
| Pathogens | HIV: Negative Hepatitis B: Negative Hepatitis C: Negative |
| Product Type | Knockout Stable Cell Line |
| Price | Inquiry |
