Cat. No.: SCELL-36239

Human FLT4 knockout ZR-75-1 cell line

Description Human FLT4 knockout ZR-75-1 cell line is edited by our special CRISPR/Cas9 technology. ZR-75-1 is a human breast cancer cell line
Species Human
Donor Disease Ductal carcinoma
Tissue/Organ/Organ System Breast; Duct; Mammary gland
Parental Cell Line ZR-75-1
Karyotype The cell line has a hypertriploid chromosome number.
Cell Morphology Epithelial cell
Growth Properties Adherent
Culture Conditions Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2.
Subculture Conditions 1:6 subculture; feed cells 2 to 3 times per week. 5% CO2 culture; 37℃
Subculture Guidelines All seeding densities should be based on cell counts obtained by established methods.
The recommended seeding density is 2*104 cells/cm2.
If necessary, a partial replacement of the medium 24 hours before subculture may help promote growth.
When the cells achieve 80-90% confluence, they should be passaged.
Viability >95% viability before freezing.
Purity Immunogen affinity purified
Quality Control HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Recommended Control Human wild-type ZR-75-1 cell line
Freezing Medium Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2.
Size 1 vial (2*106 cells/vial)
Format Frozen
Storage Vapor phase of liquid nitrogen
Shipping Info Dry Ice
Biosafety Level 1
Product Type Knockout Stable Cell Line
Knockout Validation Sanger Sequencing
Applications For research use only
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