Name: Human GPC1 knockout A549 cell line
SKU: SCELL-37073

Human GPC1 knockout A549 cell line

Description	Human GPC1 knockout A549 cell line is edited by our special CRISPR/Cas9 technology. A549 cells are squamous in nature and are responsible for the diffusion of water and electrolytes in the alveoli. When grown in vitro, these cells grow as a monolayer, adhering to the culture flask
Size	1 vial (2*10⁶ cells/vial)
Parental Cell Line	A549
Biosafety Level	1
Fromat	Frozen
Culture Conditions	Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2.
Freezing Medium	Continuous culture; DMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:4 using trypsin/EDTA;
Subculture Guidelines	All seeding densities should be based on cell counts obtained by established methods. The recommended seeding density is 2*10⁴ cells/cm². If necessary, a partial replacement of the medium 24 hours before subculture may help promote growth. When the cells achieve 80-90% confluence, they should be passaged.
Knockout Validation	Sanger Sequencing, Western Blot (WB)
Viability	>95% viability before freezing.
Adherent /Suspension	Adherent
Disease	Adenocarcinoma
Purity	Immunogen affinity purified
Storage	Vapor phase of liquid nitrogen
Sterility	Mycoplasma negative, HOECHST and PCR
Applications	For research use only
Shipping Info	Dry Ice
Species	Human
Tested Applications	Suitable for: WB
Tissue	Lung
Recommended Control	Human wild-type A549 cell line
Doubling Time	A549 cell doubling time is typically 22 hours, but can sometimes take up to 40 hours
Morphology	Epithelial, grown as monolayer
Karyotype	Hypotriploid
Pathogens	HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Cell Size	In the morphological analysis of A549 cells, the mean cell diameters from an inverted microscope and TEM images were estimated to be 14.93 µm and 10.59 µm, respectively.
Product Type	Knockout Stable Cell Line
Price	Inquiry