| Description | Human IDH1 knockout 600MPE cell line is edited by our special CRISPR/Cas9 technology. |
| Species | Human |
| Donor Disease | Invasive ductal carcinoma |
| Tissue/Organ/Organ System | Breast |
| Parental Cell Line | 600MPE |
| Cell Morphology | Epithelial cell |
| Growth Properties | Adherent |
| Culture Conditions | Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. MEM + 10% FBS + 0.01 mg/mL bovine insulin |
| Subculture Guidelines | All seeding densities should be based on cell counts obtained by established methods. The recommended seeding density is 2*104 cells/cm2. If necessary, a partial replacement of the medium 24 hours before subculture may help promote growth. When the cells achieve 80-90% confluence, they should be passaged. |
| Viability | >95% viability before freezing. |
| Purity | Immunogen affinity purified |
| Quality Control | HIV: Negative Hepatitis B: Negative Hepatitis C: Negative |
| Tested Applications | Suitable for: ICC, WB |
| Recommended Control | Human wild-type 600MPE cell line |
| Freezing Medium | 8.7% DMSO in MEM supplemented with methyl cellulose. |
| Size | 1 vial (2*106 cells/vial) |
| Format | Frozen |
| Storage | Vapor phase of liquid nitrogen |
| Shipping Info | Dry Ice |
| Biosafety Level | 1 |
| Product Type | Knockout Stable Cell Line |
| Knockout Validation | Sanger Sequencing, Western Blot (WB) |
| Applications | For research use only |
| Price | Inquiry |
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