| Description | Human IDH1 knockout 600MPE cell line is edited by our special CRISPR/Cas9 technology. |
| Size | 1 vial (2*106 cells/vial) |
| Parental Cell Line | 600MPE |
| Biosafety Level | 1 |
| Fromat | Frozen |
| Culture Conditions | Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. MEM + 10% FBS + 0.01 mg/mL bovine insulin |
| Freezing Medium | 8.7% DMSO in MEM supplemented with methyl cellulose. |
| Subculture Guidelines | All seeding densities should be based on cell counts obtained by established methods. The recommended seeding density is 2*104 cells/cm2. If necessary, a partial replacement of the medium 24 hours before subculture may help promote growth. When the cells achieve 80-90% confluence, they should be passaged. |
| Knockout Validation | Sanger Sequencing, Western Blot (WB) |
| Viability | >95% viability before freezing. |
| Adherent /Suspension | Adherent |
| Disease | Invasive ductal carcinoma |
| Purity | Immunogen affinity purified |
| Storage | Vapor phase of liquid nitrogen |
| Sterility | Bacteria: Negative Yeast: Negative Mycoplasma: Negative |
| Applications | For research use only |
| Shipping Info | Dry Ice |
| Species | Human |
| Tested Applications | Suitable for: ICC, WB |
| Tissue | Breast |
| Recommended Control | Human wild-type 600MPE cell line |
| Morphology | Epithelial cell |
| Pathogens | HIV: Negative Hepatitis B: Negative Hepatitis C: Negative |
| Product Type | Knockout Stable Cell Line |
| Price | Inquiry |
