| Description | Human LDHB (Lactate Dehydrogenase B) knockout HeLa B cell line is edited by our special CRISPR/Cas9 technology. HeLa B is a derivative of HeLa, the first aneuploid, epithelioid cell line derived from human tissue and maintained by serial subculture. |
| Size | 1 vial (2*106 cells/vial) |
| Parental Cell Line | HeLa B |
| Biosafety Level | 2 |
| Format | Frozen |
| Culture Conditions | Cultured at 37°C with 5% CO2. |
| Culture Medium | EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS) |
| Subculture Conditions | Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-3*10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA |
| Derived From | It is derived from human tissue and maintained by serial subculture |
| Knockout Validation | Sanger Sequencing, Western Blot (WB) |
| Viability | >95% viability before freezing. |
| Adherent /Suspension | Adherent |
| Disease | Carcinoma |
| Purity | Immunogen affinity purified |
| Storage | Vapor phase of liquid nitrogen |
| Sterility | All cells test negative for bacteria, yeast, and fungi. |
| Shipping Info | Dry Ice |
| Species | Human |
| Tested Applications | Suitable for: ICC, WB |
| Tissue | Cervix |
| Recommended Control | Human wild-type HeLa B cell line |
| Morphology | Epithelial |
| Pathogens | HIV: Negative Hepatitis B: Negative Hepatitis C: Negative |
| Applications | Tumourigenicity and virus studies: poliovirus 1 and Ad 3 |
| Product Type | Knockout Stable Cell Line |
| Price | Inquiry |
