| Description | Human MED12 knockout MCF-10A cell line is edited by our special CRISPR/Cas9 technology. MCF 10A is an epithelial cell line isolated in 1984 from the mammary gland of a 36-year-old Caucasian woman with fibrocystic breasts. |
| Size | 1 vial (2*106 cells/vial) |
| Parental Cell Line | MCF-10A |
| Biosafety Level | 1 |
| Format | Frozen |
| Culture Conditions | Culture at 5% CO2, 37℃ |
| Culture Medium | DMEM/F12 (1:1) + 5% Horse Serum + EGF (20 ng/mL) + Hydrocortisone (0.5 ug/mL) + Cholera Toxin (100 ng/mL) + Insulin (10ug/mL). |
| Subculture Conditions | A subculture ratio of 1:3 to 1:4 is recommended. Renew the medium every 2 to 3 days, depending on cell density. |
| Freezing Medium | 70% growth medium supplemented with 10% (v/v) DMSO and 20% horse serum |
| Knockout Validation | Sanger Sequencing, Western Blot (WB) |
| Viability | >95% viability before freezing. |
| Adherent /Suspension | Adherent |
| Disease | Fibrocystic disease |
| Purity | Immunogen affinity purified |
| Storage | Vapor phase of liquid nitrogen |
| Sterility | Mycoplasma negative, HOECHST and PCR |
| Shipping Info | Dry Ice |
| Species | Human |
| Tested Applications | Suitable for: WB |
| Tissue | Breast; Mammary gland |
| Recommended Control | Human wild-type MCF-10A cell line |
| Morphology | Epithelial |
| Pathogens | HIV: Negative Hepatitis B: Negative |
| Applications | For research use only |
| Product Type | Knockout Stable Cell Line |
| Price | Inquiry |
