| Description | It is edited by our special CRISPR/Cas9 technology. |
| Sequence Similarities | It belongs to the protein kinase superfamily. |
| Species | Human |
| Donor Disease | Carcinoma |
| Tissue/Organ/Organ System | Kidney |
| Parental Cell Line | HEK-293T cell line |
| Growth Properties | Adherent |
| Culture Conditions | Cells are cultured as a suspension at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. |
| Subculture Guidelines | The recommended seeding density is 2*104 cells/cm2. If necessary, a partial replacement of the medium 24 hours before subculture may help promote growth. When the cells achieve 80-90% confluence, they should be passaged. |
| Viability | >95% viability before freezing. |
| Purity | Immunogen affinity purified |
| Mycoplasma Free | Yes |
| Quality Control | All cells test negative for bacteria, yeast, and fungi. |
| Tested Applications | Suitable for: WB |
| Application Notes | WB; Use at an assay dependent concentration. Predicted molecular weight: 155 kDa. |
| Recommended Control | Human wild-type HEK-293T cell line |
| Recommended Culture Medium And Supplement | Growth Medium: IMDM, 10% FCS. |
| Freezing Medium | Freeze Medium: IMDM, 20% FCS, 10% DMSO |
| Size | 1 vial (2*106 cells/vial) |
| Storage | Store in liquid nitrogen. |
| Storage Buffer | Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether |
| Shipping Info | Dry Ice |
| Cell Revival | Revival: Rapidly thaw cells in a 37°C water bath. Transfer the contents to the tube containing the preheating medium. Centrifuge the cells and seeds into the pre-warmed medium in a 10 ml Petri dish. |
| Biosafety Level | 2 |
| Product Type | Knockout Stable Cell Lines |
| Knockout Validation | Sanger Sequencing, Western Blot (WB) |
| Application | For research use only |
| Price | Inquiry |
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