| Description | Human RET knockout MDA-MB-453 cell line is edited by our special CRISPR/Cas9 technology. MDA-MB-453 is a human breast cancer cell line. Cells have an active glycerol 3-phosphate shuttle. |
| Size | 1 vial (2*106 cells/vial) |
| Parental Cell Line | MDA-MB-453 |
| Biosafety Level | 1 |
| Format | Frozen |
| Culture Conditions | Cells are adherent cells that are cultured at 37℃ in air. |
| Culture Medium | L-15 + 10% FBS + 2mM L-Glutamine |
| Properties | Fibroblast growth factor (FGF) expression |
| Subculture Conditions | Split confluent culture at 1:2 to 1:4 using trypsin/EDTA; |
| Derived From | Pleural effusion |
| Freezing Medium | Culture medium + 50% FBS + 10% DMSO |
| Subculture Guidelines | All seeding densities should be based on cell counts obtained by established methods. Seed out at 4*106 cells/80 cm2 at 37℃, no exposure to CO2 (close the flask caps) about 25-35*106 cells/80 cm2 |
| Knockout Validation | Sanger Sequencing, Western Blot (WB) |
| Viability | >95% viability before freezing. |
| Adherent /Suspension | Adherent |
| Disease | Carcinoma; Metastatic |
| Purity | Immunogen affinity purified |
| Storage | Vapor phase of liquid nitrogen |
| Sterility | Mycoplasma negative, HOECHST and PCR |
| Shipping Info | Dry Ice |
| Species | Human |
| Tested Applications | Suitable for: WB |
| Tissue | Breast; Mammary gland |
| Recommended Control | Human wild-type MDA-MB-453 cell line |
| Doubling Time | ~26-38 h |
| Morphology | Epithelial, cells round in suspension, grown semiadherent |
| Pathogens | HIV: Negative Hepatitis B: Negative Hepatitis C: Negative |
| Tumorigenicity | No, in immunosuppressed mice.Yes, in semisolid medium. |
| Applications | For research use only |
| Product Type | Knockout Stable Cell Line |
| Price | Inquiry |
