| Description | Human SMARCC1 knockout MDA-MB-436 cell line is edited by our special CRISPR/Cas9 technology. MDA-MB-436 cells were derived from the pleural effusion of a 43-year-old woman with breast adenocarcinoma; MDA-MB-436 cells are pleomorphic, and most of the cells exhibited strong reaction of binding to anti-microtubule antibodies on indirect immunofluorescence staining. |
| Size | 1 vial (2*106 cells/vial) |
| Parental Cell Line | MDA-MB-436 |
| Biosafety Level | 1 |
| Format | Frozen |
| Culture Conditions | Cells are adherent cells that are cultured at 37℃ with 5% CO2. |
| Culture Medium | RPMI 1640 + 10% FBS + 2mM L-Glutamine |
| Subculture Conditions | Split cultures 1:2-1:5 using trypsin/EDTA |
| Derived From | Pleural effusion |
| Freezing Medium | Culture medium 45% + 50% FCS + 5% DMSO |
| Knockout Validation | Sanger Sequencing, Western Blot (WB) |
| Viability | >95% viability before freezing. |
| Adherent /Suspension | Adherent |
| Disease | Adenocarcinoma |
| Purity | Immunogen affinity purified |
| Storage | Vapor phase of liquid nitrogen |
| Sterility | Mycoplasma negative, HOECHST and PCR |
| Shipping Info | Dry Ice |
| Species | Human |
| Tested Applications | Suitable for: WB |
| Tissue | Breast; Mammary gland |
| Recommended Control | Human wild-type MDA-MB-436 cell line |
| Morphology | Pleomorphic with multinucleated component cells, grown as monolayer |
| Pathogens | HIV: Negative Hepatitis B: Negative Hepatitis C: Negative |
| Tumorigenicity | No, in immunosuppressed mice. Yes, in semisolid medium. |
| Applications | For research use only |
| Product Type | Knockout Stable Cell Line |
| Price | Inquiry |
