Name: Human wild-type SkBr3 cell line
SKU: SCELL-36130

Human wild-type SkBr3 cell line

Description	Human wild-type SkBr3 cell line is edited by our special CRISPR/Cas9 technology. SK-BR-3 cells were isolated in 1970 from pleural effusion cells of a 43-year-old, White, female adenocarcinoma patient with blood type A+ who had been treated with radiation, steroids, cytoxan, and 5-fluorouracil.
Size	1 vial (2*10⁶ cells/vial)
Parental Cell Line	SkBr3
Biosafety Level	1
Fromat	Frozen
Culture Conditions	Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2.
Subculture Conditions	1:2 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37℃
Freezing Medium	Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2.
Subculture Guidelines	All seeding densities should be based on cell counts obtained by established methods. The recommended seeding density is 2*10⁴ cells/cm². If necessary, a partial replacement of the medium 24 hours before subculture may help promote growth. When the cells achieve 80-90% confluence, they should be passaged.
Knockout Validation	Sanger Sequencing
Viability	>95% viability before freezing.
Adherent /Suspension	Adherent
Disease	Ductal carcinoma
Purity	Immunogen affinity purified
Storage	Vapor phase of liquid nitrogen
Sterility	Bacteria: Negative Yeast: Negative Mycoplasma: Negative
Applications	For research use only
Shipping Info	Dry Ice
Species	Human
Tissue	Breast; Mammary gland
Recommended Control	Human wild-type SkBr3 cell line
Doubling Time	Ca. 2-3 days
Morphology	Epithelial cell
Karyotype	This is a hypertriploid human cell line with 84 chromosomes
Pathogens	HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Product Type	Knockout Stable Cell Line
Price	Inquiry