| Description | During the immortalization process, the cell line is in a proliferative undifferentiated progenitor-like state, which is associated with increased levels of Polycomb Group (PcG) transcripts. |
| Tissue/Organ/Organ System | Reproductive |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | (1) Inducing muscle cell differentiation by treating cells with ecdysone and perform immunofluorescence staining and qPCR to assess the upregulated expression of mhc and Tropomyosin (Tm). (2) Up-regulated genes in DPCs are analyzed by DAVID (the Database for Annotation, Visualization and Integrated Discovery). |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Seeding Density | Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions. |
| Applications | For Research Use Only |
| Cell Morphology | Spindle shaped |
| Immortalization Method | Serial passaging of primary cultures from Act5C > UAS-RasV12 embryos |
| Price | Inquiry |
