Cat. No.: ICELL-0434

Immortalized Human Adipose-derived Stromal Cells - Bmi-1/hTERT

Description Mesenchymal stem cells have the ability to maintain pluripotency, providing a new place for cell therapy to repair damaged or diseased tissues.
Donor Gender Female
Donor Age 30
Tissue/Organ/Organ System Adipose
Immortalization Method Serial passaging and transduction with recombinant lentiviruses carrying mouse Bmi-1 (pLOX-CWBmi1) [Ref. seq.: NM_007552.4] and hTERT (pLOX-TERT-iresTK) [Ref.seq.: NM_198253.2] gene
Growth Properties Adherent
Seeding Density 6,000 - 8,000 cells/cm2
Population Doubling Time 90 - 100 Hours
Expression Profile CD44, CD73, CD90 and CD105
Quality Control 1. Real Time PCR, immunofluorescence, and telemorase activity was used to quantify Bmi-1 and/or hTERT gene expression in the immortalized cell line. 2. Culture tested negative for mycoplasma
Size 1*106 cells/1.0 ml
Format Frozen
Storage Liquid Nitrogen
Shipping Info Dry Ice
Categories Immortalized Cells
Applications For Research Use Only
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