| Description | These cells also showed the ability to differentiate into adipocytes and osteoblasts. |
| Tissue/Organ/Organ System | Bone Marrow |
| Immortalization Method | Serial passaging and transduction with recombinant retroviruses carrying hTERT gene (pBabe-puro-hTERT) |
| Cell Morphology | Spindle shaped |
| Growth Properties | Adherent |
| Seeding Density | 20,000 cells/cm2 |
| Population Doubling Time | 45 - 55 hours |
| Expression Profile | CD44, NT5E, THY1, ENG, CD90 |
| Quality Control | (1) Human telomerase reverse transcriptase expression was verified with quantitative PCR; (2) Surface markers were determined with flow cytometry; (3) Gene expression profile was analysed using the Illumina WG-6 v2 Expression BeadChip and Illumina HumanHT12 v4; (4) Adiopocyte differentiation was detected with Oil Red O staining, while osteoblast differentiation was detected by staining cells with Alizarin Red and ALPL and quantification; (5) DNA methylation profiling was performed(6) Karyotype analysis was performed by arresting cells during metaphase and subjecting chromosomes to G-banding and subsequent imaging; (7) Tumorgenicity was verified in vivo with subcutaneous injection into immunodeficient athymic mice; (8) miRNA expression profile was determined using high-throughput sequencing. (9) Puromycin selection used (2 μg/mL) however not needed for long term culture. |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Storage | Liquid Nitrogen |
| Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
| Shipping Info | Dry Ice |
| Categories | Immortalized Cells |
| Applications | For Research Use Only |
| Price | Inquiry |
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