| Description | Immortalized human brown preadipocytes are derived from infant brown adipose tissue. |
| Tissue/Organ/Organ System | Adipose |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | 1) Compared preadipocyte and adipocyte expression after terminal differentiation via semi-quantitative RT-PCR 2) Visualized morphology of differentiated and undifferentiated cells via phase contrast microscopy 3) Compared the expression profiles of primary and differentiated t0729 cells |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Seeding Density | 10,000 - 30,000 cells/cm2 |
| Applications | For Research Use Only |
| Cell Morphology | Epithelial |
| Donor Age | Infant |
| Expression Profile | Positive for Proliferator activated receptor gamma (PPARγ) and Uncoupling protein 1. Negative for human fatty acid binding protein (aP2), lipoprotein lipase (LPL), β3-adrenergic receptor (β3-AR) and hormone sensitive lipase (HSL) |
| Immortalization Method | SV40 large and small T-antigen |
| Population Doubling Time | 90 - 100 hours |
| Price | Inquiry |
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