| Description | Due to their multiple differentiation capabilities, mesenchymal stromal cells (MSCs) have great potential in tissue engineering and regenerative medicine, and show important clinical potential in cell therapy. |
| Tissue/Organ/Organ System | Reproductive |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | RT-PCR was used to confirm the expression of the hTERT transgene in the immortalized cells; 2) TRAP assay was performed to determine the telomerase activity; 3) Flow cytometry was used to detect surface markers such as CD29, CD73, CD105, CD44 and HLA-ABC; 4) G-banding was used for karyotype analysis; 5) Tumuorigenicity assay was performed to evaluate the cells’ tumorigenic potential. |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Seeding Density | 30,000 - 40,000 cells/cm2; Split ratio of 1:3 to 1:5 |
| Applications | For Research Use Only |
| Cell Morphology | Fibroblast-like |
| Donor Gender | Female |
| Expression Profile | CD29, CD73, CD105, CD44 and HLA-ABC |
| Immortalization Method | Serial passaging and transfection with Human Telomerase Reverse Transcriptase (hTERT) expression vector pGRN145 |
| Population Doubling Time | Population doubling time of 23.8 ± 1.9 h |
| Price | Inquiry |
