Cat. No.: ICELL-0076

Immortalized Human Cord Blood Mesenchymal Stromal Cells with RFP (cbMSC-hTERT-RFP)

Donor Gender Female
Tissue/Organ/Organ System Reproductive
Immortalization Method Serial passaging and transfection with Human Telomerase Reverse Transcriptase (hTERT) expression vector pGRN145
Cell Morphology Fibroblast-like
Growth Properties Adherent
Population Doubling Time Population doubling time of 23.8 ± 1.9 h
Expression Profile CD90, CD34, CD44, CD45, CD166, CD117,CD73, CD105, CK18 and CK19
Quality Control RT-PCR was used to confirm the expression of the hTERT transgene in the immortalized cells; 2) TRAP assay was performed to determine the telomerase activity; 3) Flow cytometry was used to detect surface markers such as CD29, CD73, CD105, CD44 and HLA-ABC; 4) G-banding was used for karyotype analysis; 5) Tumuorigenicity assay was performed to evaluate the cells’ tumorigenic potential.
Size 1*106 cells/1.0 ml
Format Frozen
Storage Liquid Nitrogen
Shipping Info Dry Ice
Categories Immortalized Cells
Applications For Research Use Only
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