| Description | The function of the corneal endothelium is to regulate corneal hydration. The dysfunction of this key cell layer will gradually lead to corneal opacity, eventually leading to vision loss and corneal blindness. |
| Tissue/Organ/Organ System | Eye |
| Immortalization Method | Serial passaging and transduction with retroviruses carrying human papillomavirus type 16 E6/E7 gene |
| Cell Morphology | Cobble-stone |
| Growth Properties | Adherent |
| Seeding Density | 20, 000 cells/cm2; Split ratio of 1:2 to 1:3 |
| Population Doubling Time | 54 hours |
| Expression Profile | CX43, ZO-1, 9.3E |
| Quality Control | RT-PCR was used to evaluate the transgene expression of HPV16 E6/E7; 2) Indirect immunofluorescence was performed to detect the cell specific marker expression CX43, ZO-1 and 9.3E; 3) FISH was used to examine the cytogenetic alterations in the immortalized cells. |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Storage | Liquid Nitrogen |
| Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
| Shipping Info | Dry Ice |
| Categories | Immortalized Cells |
| Applications | For Research Use Only |
| Price | Inquiry |
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