| Description | Corneal keratinocytes play an important role in maintaining the balance of matrix materials and maintaining corneal transparency. |
| Tissue/Organ/Organ System | Eye |
| Immortalization Method | Serial passaging and transduction with retroviruses carrying human papillomavirus type 16 E6/E7 gene |
| Cell Morphology | Polygonal |
| Growth Properties | Adherent |
| Seeding Density | 30,000 - 50,000 cells/cm2 |
| Population Doubling Time | 24 hours |
| Expression Profile | CK19, CK12 |
| Quality Control | RT-PCR was used to evaluate the transgene expression of HPV16 E6/E7; 2) Indirect immunofluorescence was performed to detect the cell specific marker expression CK12, CK19 and ABCG2; 3) FISH was used to examine the cytogenetic alterations in the immortalized cells. |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Storage | Liquid Nitrogen |
| Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
| Shipping Info | Dry Ice |
| Categories | Immortalized Cells |
| Applications | For Research Use Only |
| Price | Inquiry |
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