| Description | Immortalized human skull periosteum cells are the first human skull periosteum cell line isolated from the biopsy of healthy adult patients, and immortalized with large SV40 T antigen through lentiviral transduction. |
| Tissue/Organ/Organ System | Bone |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | 1) Osteogenic differentiation experiments; 2) Western blot; 3) Flow cytometry; 4) Detection of cell mineralization by fluorescent OsteoImage staining; 5) Gene expression analysis |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Applications | For Research Use Only |
| Cell Morphology | Polygonal |
| Expression Profile | CD44, CD73, CD90, CD105, CD146, CD166, MSCA-1 and low expression of CD34; neomycin resistance (0.25 mg/mL G418) |
| Immortalization Method | Immortalized with lentivirus containing large SV40 T antigen |
| Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
| Price | Inquiry |
