| Description | Cervical epithelium lines the cervix of the female reproductive tract, separating sterile and microbial-rich areas from each other |
| Tissue/Organ/Organ System | Reproductive |
| Immortalization Method | Serial passaging and transduction with retroviruses carrying Human papillomavirus E6/E7 (type 16) protein (pLXSN) |
| Cell Morphology | Cobble-stone |
| Growth Properties | Adherent |
| Seeding Density | Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions. |
| Expression Profile | MUC5B |
| Quality Control | (1) Electron microscopy was performed to assess cell polarization, while fluorescent microscopy was performed to assess tight junction formation between cells. (2) Transepithelial electrical resistance was measured to measure cell monolayer functional integrity. (3) RT-PCR, Western blotting, and immunostaining were performed to compare immortalized cell characteristics to normal counterparts. (4) Expression levels of antimicrobial peptides, cytokines, and chemokines were measured to further assess immortalized cell characteristics. (5) qPCR was performed to analyze toll-like receptor protein expression between basolateral and apical components of cells, while qRT-PCR was performed to determine hormone receptor functionality. |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Storage | Liquid Nitrogen |
| Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
| Shipping Info | Dry Ice |
| Categories | Immortalized Cells |
| Applications | For Research Use Only |
| Price | Inquiry |
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