Cat. No.: ICELL-0201

Immortalized Human Fetal Liver Cells (cBAL111)

Description Human fetal hematopoietic cells are immortalized by stable lentiviral overexpression of human telomerase reverse transcriptase (hTERT).
Tissue/Organ/Organ System Liver
Immortalization Method Immortalized with lentivirus containing hTERT
Cell Morphology Spindle-shaped
Growth Properties Adherent
Expression Profile Ampicillin & neomycine-resistant, expresses albumin, glutamin synthetase, cytokeratin 18, and cytokeratin 19.
Quality Control 1) qRT-PCR for hTERT expression; 2) cBAL111 clone continued to proliferate whereas primary cells senescenced in culture; 3) Immunostaining conducted showed cells are positive for hepatic markers albumin, glutamin synthetase and cytokeratin 18, and positive for cholangiocyte marker cytokeratin 19.
Size 1*106 cells/1.0 ml
Format Frozen
Storage Liquid Nitrogen
Preservation Protocol 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid Nitrogen vapour phase.
Shipping Info Dry Ice
Categories Immortalized Cells
Applications For Research Use Only
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