Cat. No.: ICELL-0063

Immortalized Human Granulosa Cells (HGL5)

Description Human ovarian granulosa cells are the place where estrogen and progesterone are produced. Therefore, it is of great significance to study steroid biosynthesis, steroid metabolism enzymes, specific growth factors and gonadotropins.
Tissue/Organ/Organ System Ovary
Immortalization Method Human papilloma virus strain 16 (HPV16) oncogenes E6/E7
Cell Morphology Polygonal
Growth Properties Adherent
Quality Control 1) Expression of viral genes quantified by PCR; 2) cAMP, progesterone and estradiol production were measured after induction with forskolin, dbcAMP, follicle-stimulating hormone (FSH), or luteinizing hormone (LH) treatments; 3) p450arom regulation characterized via North blot analysis
Size 1*106 cells/1.0 ml
Format Frozen
Storage Liquid Nitrogen
Shipping Info Dry Ice
Categories Immortalized Cells
Applications For Research Use Only
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