| Description | DPC is a useful tool for studying the regulation of hair growth. |
| Donor Disease | Donor Info Not Disclosed |
| Donor Gender | Donor Info Not Disclosed |
| Donor Age | Donor Info Not Disclosed |
| Donor Ethnicity | Donor Info Not Disclosed |
| Tissue/Organ/Organ System | Hair Follicle |
| Immortalization Method | Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen and c-Myc gene |
| Cell Morphology | Flat|Polygonal |
| Growth Properties | Adherent |
| Seeding Density | 20,000 - 30,000 cells/cm2, Recommended Split ratio of 1:2 |
| Expression Profile | Smooth muscle alpha actin, alkaline phosphatase |
| Quality Control | 1) Western blot was used to identify SV40T and c-myc transgene expression. 2) Immunocytochemistry and western blot were performed to assess the expression of smooth muscle actin alpha and alkaline phosphatase. 3) Up-regulated genes in DPCs is analyzed by DAVID (the Database for Annotation, Visualization and Integrated Discovery). |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Storage | Liquid Nitrogen |
| Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
| Shipping Info | Dry Ice |
| Categories | Immortalized Cells |
| Applications | For Research Use Only |
| Price | Inquiry |
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