Cat. No.: ICELL-0168

Immortalized Human Lens Epithelial Cells - SV40

Description These cells also provide a unique system to examine the relationship between gene expression and differentiation.
Tissue/Organ/Organ System Eye
Immortalization Method Serial passaging and transduction with recombinant adenovirus carrying SV40 Large T antigen
Cell Morphology Polygonal
Growth Properties Adherent
Seeding Density 5,000 - 10,000 cells/cm2; Recommended split ratio is 1:2
Population Doubling Time 76 hours
Quality Control Immortalized human lens epithelial cells reacted positively with monoclonal antibodies against the SV40 large T antigen. Western blotting and precipitation are used to confirm the presence of βH- and γ-crystallin, which are markers of lens epithelial cell differentiation.
Size 1*106 cells/1.0 ml
Format Frozen
Storage Liquid Nitrogen
Shipping Info Dry Ice
Categories Immortalized Cells
Applications For Research Use Only
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