| Description | HMF3S cells grow best at 37⁰C and are insensitive to temperature compared to immortalized human mammary fibroblasts (HMF3A) |
| Tissue/Organ/Organ System | Mammary |
| Immortalization Method | Combined transduction with retroviruses carrying a temperature sensitive mutant (U19tsA58) of the SV40 Large T Antigen and a catalytic subunit of human telomerase (hTERT) |
| Cell Morphology | Spindle shaped |
| Growth Properties | Adherent |
| Seeding Density | 10,000 - 15,000 cells/cm2 |
| Population Doubling Time | 45 hours |
| Quality Control | 1) Telomerase-detection assay was used to detect telomerase activity. 2) Flow cytometry confirmed expression of T antigen. |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Storage | Liquid Nitrogen |
| Shipping Info | Dry Ice |
| Categories | Immortalized Cells |
| Applications | For Research Use Only |
| Price | Inquiry |
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