Cat. No.: ICELL-0230

Immortalized Human Neonatal Foreskin Keratinocytes (HKc16E6/E7-II)

Description The HKc16E6/E7-II cell line can also be used as a model to analyze the effects of HPV16 on the function and pathology of human epithelial cells.
Tissue/Organ/Organ System Skin
Immortalization Method Transfection with HPV16 E6 and E7
Cell Morphology Epithelial-like
Growth Properties Adherent
Expression Profile HPV-16 E6/E7 expression; neomycin resistance; and
ampicillin resistance
Quality Control 1) RNaseH assays; 2) ODN uptake measurements; 3) Western blot; 4) RT-PCR
Size 1*106 cells/1.0 ml
Format Frozen
Storage Liquid Nitrogen
Preservation Protocol 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid Nitrogen vapour phase.
Shipping Info Dry Ice
Categories Immortalized Cells
Applications For Research Use Only
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