Cat. No.: ICELL-0195

Immortalized Marmoset Hepatic Progenitor Cells

Description Immortalized marmoset liver progenitor cells (MHPC) are produced by lentivirus-mediated transfer of the 40 large T antigen genes of monkey virus into fetal liver polygonal cells.
Tissue/Organ/Organ System Liver
Immortalization Method Immortalized with lentivirus containing the SV40 T antigen
Cell Morphology Elongated
Growth Properties Adherent
Population Doubling Time 55 hours
Expression Profile Hepatic progenitor markers (CD44, Sox9, Sox17 and Ncam1)
Quality Control 1) Karyotype and telomere analysis; 2) RNA sequencing; 3) Reverse transcription-PCR; 4) Immunocytochemistry and immunohistochemistry; 5) Histology; 6) PAS, ICG, and CDFDA uptake assays and Oil Red O staining; 7) In vitro differentiation; 8) CYP450 induction
Size 1*106 cells/1.0 ml
Format Frozen
Storage Liquid Nitrogen
Preservation Protocol 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid Nitrogen vapour phase.
Shipping Info Dry Ice
Categories Immortalized Cells
Applications For Research Use Only
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