| Description | Immortalized mouse astrocyte-SV40 T is a suitable cell line for studying the function of astrocytes. |
| Tissue/Organ/Organ System | Brain |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | The response of the cell line to pro-inflammatory stimuli was measured by: 1) immunostaining to assess the translocation of NF-kB from the cytoplasm to the nucleus; 2) RT-qPCR and quantitative PCR detection of NOS-2 mRNA levels; and 3 ) Western blot analysis of NOS-2 and COX2 proteins. |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Seeding Density | 5,000 - 10,000 cells/cm2 |
| Applications | For Research Use Only |
| Cell Morphology | Stellate shaped |
| Expression Profile | Nestin, CD44 |
| Immortalization Method | Serial passaging and transfection with psV3neo plasmid carrying SV40 large T antigen genes |
| Population Doubling Time | 20 - 30 hours |
| Price | Inquiry |
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