Cat. No.: ICELL-0416

Immortalized Mouse Astrocytes - SV40T (IMA2.1)

Description Immortalized mouse astrocyte-SV40 T is a suitable cell line for studying the function of astrocytes.
Tissue/Organ/Organ System Brain
Immortalization Method Serial passaging and transfection with psV3neo plasmid carrying SV40 large T antigen genes
Cell Morphology Stellate shaped
Growth Properties Adherent
Seeding Density 5,000 - 10,000 cells/cm2
Population Doubling Time 20 - 30 hours
Expression Profile Nestin, CD44
Quality Control The response of the cell line to pro-inflammatory stimuli was measured by: 1) immunostaining to assess the translocation of NF-kB from the cytoplasm to the nucleus; 2) RT-qPCR and quantitative PCR detection of NOS-2 mRNA levels; and 3 ) Western blot analysis of NOS-2 and COX2 proteins.
Size 1*106 cells/1.0 ml
Format Frozen
Storage Liquid Nitrogen
Shipping Info Dry Ice
Categories Immortalized Cells
Applications For Research Use Only
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