Cat. No.: ICELL-0182

Immortalized Mouse Cardiac Endothelial Cells (MCEC)

Description Immortalized mouse atrioventricular mat mesenchymal cells (tsA58-AVM) were derived from the atrioventricular (AV) mat of E9.5 H-2Kb-tsA58 embryos and conditionally immortalized in subsequent cultures.
Tissue/Organ/Organ System Heart
Immortalization Method Immortalized via infection with lentivirus containing hTERT and SV40-T antigen
Cell Morphology Endothelial-like
Growth Properties Adherent
Seeding Density 15,000 - 20,000 cells/cm2
Population Doubling Time 20 - 30 hours
Expression Profile SV40-T, hTERT, PECAM-1, VE-cadherin, von Willebrand Factor
Quality Control 1) Cell incubation and prostaglandin assays; 2) Immunofluorescence studies; 3) Western blot; 4) rtPCR; 5) Immunohistochemistry; 6) Permeability studies
Size 1*106 cells/1.0 ml
Format Frozen
Storage Liquid Nitrogen
Shipping Info Dry Ice
Categories Immortalized Cells
Applications For Research Use Only
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