| Description | Immortalized mouse CD4+ CD8+ T cells have the characteristics of an immortalized cell line, but they will not be immortalized by any immortalizing reagents. |
| Tissue/Organ/Organ System | Blood |
| Immortalization Method | Spontaneous immortalization |
| Cell Morphology | Round |
| Growth Properties | Suspension |
| Seeding Density | 400,000 - 800,000 cells/ml; Recommended split ratio is 1:2 to 1:5 |
| Population Doubling Time | 25 - 35 hours |
| Quality Control | 1) Flow cytometry to analyze CD4+ CD8+ phenotype; 2) PCR assay for T cell receptor expression; 3) Sequence analysis to identify point mutations in Notch1 and Jak1 |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Storage | Liquid Nitrogen |
| Preservation Protocol | Freeze in Prigrow II with 20% fetal bovine serum (TM999) and 5% DMSO |
| Shipping Info | Dry Ice |
| Categories | Immortalized Cells |
| Applications | For Research Use Only |
| Price | Inquiry |
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