| Description | YAMC is a conditionally immortalized mouse colonic epithelial cell line, which is isolated from mice carrying a heat labile mutation (tsA58) and is affected by the interferon (IFN)-γ-inducible H-2Kb promoter And temperature-sensitive simian virus 40 large T antigen control. |
| Tissue/Organ/Organ System | Colon |
| Immortalization Method | Isolated from transgenic mouse carrying a temperature-sensitive simian virus 40 tumor antigen (tsA58) |
| Cell Morphology | Epithelial |
| Growth Properties | Adherent |
| Seeding Density | 20,000 cells/cm2. Recommended split ratio of 1:3 |
| Population Doubling Time | 22 - 32 hours |
| Expression Profile | CK18 |
| Quality Control | 1) Immunohistochemical staining was used to detect the presence of SV40 large T antigen and keratin 18; 2) Biochemical assays (i.e. method of Nagatsu et al and method of Dahlquist) were used to measure brush border-associated enzymes alkaline phosphatase, dipeptidyl peptidase IV and sucrase. |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Storage | Liquid Nitrogen |
| Shipping Info | Dry Ice |
| Categories | Immortalized Cells |
| Applications | For Research Use Only |
| Price | Inquiry |
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