Cat. No.: ICELL-0454

Immortalized Mouse Conditionally Reprogrammed Otic Stem Cells (CR-OSC)

Description Ear stem cells are auditory progenitor cells that can differentiate into cells that are similar to auditory hair cells (HC) and express the HC gene.
Tissue/Organ/Organ System Auditory
Immortalization Method Conditional reprogramming
Cell Morphology Polygonal
Growth Properties Adherent
Expression Profile Prestin, Atoh1, Pou4f3, myosin VI, myosin VIIa, parvalbumin, otoferlin, and VGlut3
Quality Control 1) Immunohistochemistry to detect the up-regulation of gene response to differentiation conditions; 2) Atoh1 overexpression virus transduction to detect gene expression response; 3) Thyroid hormone therapy to detect gene expression response
Size 1*106 cells/1.0 ml
Format Frozen
Storage Liquid Nitrogen
Preservation Protocol 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid Nitrogen vapour phase.
Shipping Info Dry Ice
Categories Immortalized Cells
Applications For Research Use Only
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