| Description | The wild-type MutuDC1940 cell line is an immortalized splenic CD8α+ subset of conventional dendritic cells from CD11c:SV40LgT transgenic mice. |
| Tissue/Organ/Organ System | Spleen |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | 1) Direct antigen presentation and cross-antigen presentation were evaluated by the MHC-I (SIINFEKL/OT-I ) and MHC-II (OVA323-339/OT-II) restricted systems; 3) proteome profile and surface markers assessed by RT-PCR; RT-PCR; 3) IL-12 cytokine secretion analyzed using ELISA ; 4) Response to PAMP stimulation evaluated by functional assays |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Seeding Density | 20,000 – 60,000 cells/cm2. Recommended split ratio: no greater than 1:3. |
| Applications | For Research Use Only |
| Cell Morphology | Small aggregates |
| Expression Profile | CD11c, CD24, MHC-II+, DEC205, Clec9A, B220, CD11b, IRF4, IRF8, CD4 |
| Immortalization Method | Isolated from C57BL/6 transgenic mice carrying SV40 Large T oncogene |
| Population Doubling Time | 45 - 55 hours |
| Price | Inquiry |
