Cat. No.: ICELL-0265

Immortalized Mouse Dental Apical Papilla Progenitor Cells (iSCAP)

Description It is recommended that these cells be used in research involving apical papilla stem cell biology and dental tissue engineering.
Tissue/Organ/Organ System Oral
Immortalization Method Serial passaging and transduction with retrovirus SSR#69 expressing SV40 large T antigen flanked with Cre/loxP sites
Cell Morphology Polygonal
Growth Properties Adherent
Seeding Density Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Expression Profile CD73, CD44, CD90/Thy-1, CD105/Endoglin, CD166/ALCAM, CD133/Prom1, BMPRII, CD117/c-kit, and CD29/integrin β1
Quality Control 1) Immunofluorescence staining performed to examine mesenchymal stem cell markers; 2) sqPCR analysis for BMP9-induced late osteo/odontoblast, and ondotoblast markers.
Size 1*106 cells/1.0 ml
Format Frozen
Storage Liquid Nitrogen
Shipping Info Dry Ice
Categories Immortalized Cells
Applications For Research Use Only
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