| Description | Immortalized mouse embryonic liver progenitor cells are non-tumorigenic, hygromycin-resistant, and reversible immortalized cells that can express endothelial (CD34), progenitor cells (Pou5f1/Oct4) and liver (AFP, Dlk, Alb) cell markers . |
| Tissue/Organ/Organ System | Liver |
| Immortalization Method | Serial passaging and transduction with retrovirus SSR#69 expressing SV40 large T antigen flanked with Cre/loxP sites |
| Cell Morphology | Polygonal |
| Growth Properties | Adherent |
| Seeding Density | 10,000 - 30,000 cells/cm2 |
| Population Doubling Time | 30 - 40 hours |
| Expression Profile | CD34, Pou4f1/Oct4, AFP, Dlk, Alb |
| Quality Control | 1) qPCR to detect the expression of cell markers; 2) Western blot analysis of SV40 large T antigen; 3) Indocyanine green (ICG) uptake and release assay and periodic acid-Schiff (PAS) staining to determine cell function. |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Storage | Liquid Nitrogen |
| Shipping Info | Dry Ice |
| Categories | Immortalized Cells |
| Applications | For Research Use Only |
| Price | Inquiry |
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