Cat. No.: ICELL-0385

Immortalized Mouse Hepatic Stellate Cells - SV40

Description Immortalized mouse hepatic stellate cells-SV40 from C57/Bl6 WT mice, digested by enzyme and centrifuged with Percoll density gradient.
Tissue/Organ/Organ System Liver
Immortalization Method Transfection of pCMV Simian Virus 40 Large T antigen
Cell Morphology Polygonal
Growth Properties Adherent
Seeding Density 20,000 - 40,000 cells/cm2
Population Doubling Time 35 - 45 hours
Quality Control 1) Genotype analysis; 2)NF-κB Activation Assay; 3) Cytokine ELISA Assays; 4) 3[H]-thymidine Cell Proliferation Assay
Size 1*106 cells/1.0 ml
Format Frozen
Storage Liquid Nitrogen
Preservation Protocol 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid Nitrogen vapour phase.
Shipping Info Dry Ice
Categories Immortalized Cells
Applications For Research Use Only
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