| Description | Immortalized mouse hepatic stellate cells-SV40 from C57/Bl6 WT mice, digested by enzyme and centrifuged with Percoll density gradient. |
| Tissue/Organ/Organ System | Liver |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | 1) Genotype analysis; 2)NF-κB Activation Assay; 3) Cytokine ELISA Assays; 4) 3[H]-thymidine Cell Proliferation Assay |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Seeding Density | 20,000 - 40,000 cells/cm2 |
| Applications | For Research Use Only |
| Cell Morphology | Polygonal |
| Immortalization Method | Transfection of pCMV Simian Virus 40 Large T antigen |
| Population Doubling Time | 35 - 45 hours |
| Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
| Price | Inquiry |
