Cat. No.: ICELL-0240

Immortalized Mouse Late Stage Melanocytes (iMC65)

Description These cells are valuable tools for the study of melanocyte biology and the molecular pathogenesis of pigment-related cell diseases.
Tissue/Organ/Organ System Skin
Size 1*106 cells/1.0 ml
Format Frozen
Shipping Info Dry Ice
Quality Control 1) Fontana-Masson staining for melanocytic cell markers; 2) Tyrosine enzymatic assays determined presence of tyrosine; 3) Western blot analysis for SV40 large T antigen presence
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Categories Immortalized Cells
Seeding Density Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications For Research Use Only
Cell Morphology Polygonal
Expression Profile Pax3, Sox10, MITF-m, c-kit
Immortalization Method Serial passaging and transduction with retrovirus SSR#69 expressing SV40 large T antigen flanked with Cre/loxP sites
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